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Nonetheless, results such as inflammatory biomarkers, gene expression and cytotoxicity from either in vitro studies where the experimental model used to assess the safety of EC flavors closely recapitulates the microenvironments of the 3D human lung (ALI, or lung-on-chip) or in vivo animal testing may help discuss the potential extrapolation of EC toxicity to humans, taking into consideration dosage and exposure times required to mimic human exposures (Figure 3)